Pharmaceutical Microbiology Laboratory Manual

LIST OF EXPERIMENTS

1. Study of equipment and apparatus used in experimental microbiology

2. Sterilization of glass ware

3. Preparation of media (nutrient stabs and slants preparations) and sterilization

4. Sub culturing of bacteria and fungus

5. Staining techniques – Simple staining; Gram’s staining; Negative staining; Acid-fast staining

6. Study of motility characters

7. Study of the methods of isolation of pure culture

8. Bio chemical testing for the identification of micro‐organisms.

9. Sterility testing for powders and liquids

10.Microbiological assay of antibiotics by cup plate method

REGULAR PRACTICAL ASSESSMENT SCHEME

Sl. no.

Parameters

Marks allotted

Maintenance of practical manual book

Record book neatness and timely

submission

Practical Skills

Result analysis

Viva voce

Attendance and discipline

Total

Laboratory Safety: Do’s andDon’ts

1. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave.

2. Absolutely no food, drinks, chewing gum is allowed in the laboratory. Do not put anything in your mouth such as pencils, pens, labels, or fingers. Do not store food in areas where microorganisms are stored.

3. Avoid loose fitting items of clothing. Wearappropriate shoes/sandals in the laboratory.

4. Keep your hands away from your mouth and eyes.

5. Keep your workspace free of all unnecessary materials. Backpacks, purses should be placed in the appropriate place.

DO NOT KEEP YOURBAGS ON WORKING TABLE.

6. Disinfect work areas before and after use with 70% ethanol or fresh 10% bleach.

7. Laboratory equipment and work surfaces should be decontaminated with an appropriate disinfectant on a routine basis, and especially after spills, splashes, or other contamination.

8. Label everything clearly.

9. Replace caps on reagents, solution bottles, and bacterial cultures. Do not open Petri dishes in the lab unless absolutely necessary.

10. Inoculating loops and needles should be flame sterilized in a Bunsen burner before you lay them down. Do not walk around in the laboratory with a loop or pipette having infectious materials.

11. Turn off Bunsen burners when not in use.

12. When you flame sterilize with alcohol, be sure that you do not have any papers under You.

13. Treat all microorganisms as potential pathogens. Use appropriate care and do not take cultures out of the laboratory.

14. Wear disposable gloves when working with potentially infectious microbes or samples. If you are working with a sample that may contain a pathogen, then be extremely careful to use good bacteriological technique.

15. Sterilize equipment and materials.

16. Never pipette by mouth. Use a pipetting aid or adjustable volume pipettes.

17. Consider everything a biohazard. Do not pour anything down the sink. Autoclave liquids and broth cultures to sterilize them before discarding.

18. Dispose of all solid waste material in a biohazard bag and autoclave it before discarding.

19. Report spills and accidents immediately to your instructor. Clean small spills with care and seek help for large spills.

20. Report all injuries or accidents immediately to the instructor, no matter how small they seem.

21. Students are not allowed to touch any equipment, chemicals or other materials in the laboratory area until you are instructed by Teacher or Technician.

22. Before starting Laboratory work follow all written and verbal instructions carefully.

23. If you do not understand a direction or part of a procedure, ASK YOUR CONCERN TEACHER BEFORE PROCEEDING WITH THE ACTIVITY.

24. Before use equipment must be read carefully Labels and instructions. Set up and use the equipment as directed by yourteacher.