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Chromatographic Conditions:

Flow Rate : 0.900 mL/min

Injection Volume : 10.00 ml

Auto Sampler Temperature : 5±3°c

Column Oven Temperature : 35±2°c

Run Time : 3.20 mins

Column : Luna Omega 3 mm Pollar C18, 100x4.6 mm

Retention Time :  Analyte  - 1.25 ± 0.5 min, ISTD - 1.25 ± 0.5 min

Pump Mode : Binary

Mobile Phase : Binary Flow (Total 0.900 mL Flow) , Mobile Phase Buffer 20%, Organic Mixture 80%

Split Ratio : 50% in flow into Mass spectrometer

 

Source Parameters

Ion Source : Turbo Spray

Polarity : Positive

CUR : 40.00

CAD : 9.00

IS : 5500.00

TEMP : 500.00

GS1 : 45.00

GS2 : 45.00

Resolution : Q1:Unit Q3:Unit

 

Compound Parameters Analyte

Scan Type : MRM

Q1 (m/z) : 226.200

Q3 (m/z) : 152.200

Dwell Time (m Sec) : 200.00

CE : 10.00

CXP : 12.00

DP : 65.00

EP : 10.00

 

Compound Parameter ISTD

Scan Type : MRM

Q1 (m/z) : 230.300

Q3 (m/z) : 152.000

Dwell Time (m Sec) : 200.00

CE : 20.00

CXP : 10.00

DP : 65.00

EP : 10.00